- Featured Product
- KD/KO Validated
FBF1 Polyclonal antibody
FBF1 Polyclonal Antibody for IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11531-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | A549 cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 19 publications below |
| IP | See 1 publications below |
Product Information
11531-1-AP targets FBF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FBF1 fusion protein Ag2106 |
| Full Name | Fas (TNFRSF6) binding factor 1 |
| Calculated Molecular Weight | 1133 aa, 125 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC023549 |
| Gene Symbol | FBF1 |
| Gene ID (NCBI) | 85302 |
| RRID | AB_2246683 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FBF1 antibody 11531-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. | ||
Nat Commun Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules. | ||
Nat Commun A distal centriolar protein network controls organelle maturation and asymmetry. | ||
Nat Commun TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating. | ||
Nat Commun Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis. |
