Back

Fibulin 5 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 12188-1-AP

Featured Product KD/KO validated

  • Print page
  • Download PDF

Be the first to review this product

-_-

Freight/Packing

Con: 56 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Special offer: Add control/tag antibody for $99
Load more

Tested Applications

Applications:
WB, IP, IHC, ELISA
Application not tested? Please see our Guarantee


Images See all

Fibulin 5 Antibody WB mouse heart tissue 12188-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP(Fibulin 5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

Fibulin 5 Antibody IHC mouse lung tissue 12188-1-AP

Immunohistochemistry of paraffin-embedded mouse lung tissue slide using 12188-1-AP( Fibulin 5 antibody at dilution of 1:200

Fibulin 5 Antibody IHC mouse lung tissue 12188-1-AP

Immunohistochemistry of paraffin-embedded mouse lung tissue slide using 12188-1-AP( Fibulin 5 antibody at dilution of 1:200

Fibulin 5 Antibody IP mouse heart tissue 12188-1-AP

IP Result of anti-Fibulin 5 (IP:12188-1-AP, 3ug; Detection:12188-1-AP 1:300) with mouse heart tissue lysate 8000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, mouse placenta tissue, rat brain tissue
Positive IP detected in:
mouse heart tissue
Positive IHC detected in:
mouse lung tissue, human heart tissue, human pancreas tissue
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Fibulin 5 fusion protein Ag2859
Full name:
fibulin 5
Calculated molecular weight:
448aa,50 kDa
Observed molecular weight:
64-66 kDa
GenBank accession number:
BC022280
Gene ID (NCBI):
10516
Gene symbol
FBLN5
Synonyms
ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein

Background

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50kd or 66kd band in western blotting, the 66kd corresponding to fully glycosylated protein.


Back
to top