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FBXO28 Polyclonal antibody

FBXO28 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24282-1-AP

Synonyms

F box only protein 28, F box protein 28, Fbx28, FBXO28, KIAA0483

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO28 Polyclonal antibody (24282-1-AP)

    WB analysis using 24282-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24282-1-AP (FBXO28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FBXO28 Polyclonal antibody (24282-1-AP)

    IHC staining of human breast cancer using 24282-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24282-1-AP (FBXO28 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FBXO28 Polyclonal antibody (24282-1-AP)

    IHC staining of human kidney using 24282-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24282-1-AP (FBXO28 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FBXO28 Polyclonal antibody (24282-1-AP)

    IHC staining of human breast cancer using 24282-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24282-1-AP (FBXO28 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FBXO28 Polyclonal antibody (24282-1-AP)

    IHC staining of human kidney using 24282-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24282-1-AP (FBXO28 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 24282-1-AP is FBXO28 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO28 fusion protein Ag14900
Full Name F-box protein 28
Calculated Molecular Weight 368 aa, 41 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC031576
Gene Symbol FBXO28
Gene ID (NCBI) 23219
RRIDAB_2879469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO28 also named as F box only protein 28 is a 368 amino-acid protein, which contain 1 F-box domain and belongs to F-box protein family. FBXO28 is a critical component of SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. FBOX28 encodes a cell cycle regulated protein with a critical function in tumor cell proliferation. FBXO28 assembles an SCF-FBXO28 ubiquitin ligase whose activity is required during progression through the cell cycle by cyclin-dependent kinase 1/2 mediated phosphorylation. FBXO28 expression and phosphorylation to be strongly associated with poor prognosis and worse overall survival in human breast cancer.

Protocols

Product Specific Protocols
WB protocol for FBXO28 antibody 24282-1-APDownload protocol
IHC protocol for FBXO28 antibody 24282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncogene

FBXO28 suppresses liver cancer invasion and metastasis by promoting PKA-dependent SNAI2 degradation

Authors - Xinran Qiao
View Article