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FCRLA Polyclonal antibody

FCRLA Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26949-1-AP

Synonyms

Fc receptor like A, Fc receptor like protein, Fc receptor related protein X, FCRL, FCRL1, FCRLA, FCRLb, FCRLc1, FCRLc2, FCRLd, FCRLe, FCRLM1, FCRLX, FCRX, FREB, RP11 474I16.5

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using FCRLA Polyclonal antibody (26949-1-AP)

    WB analysis of Raji using 26949-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26949-1-AP (FCRLA Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using FCRLA Polyclonal antibody (26949-1-AP)

    WB analysis of Daudi using 26949-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 26949-1-AP (FCRLA Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using FCRLA Polyclonal antibody (26949-1-AP)

    WB analysis of Raji using 26949-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26949-1-AP (FCRLA Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using FCRLA Polyclonal antibody (26949-1-AP)

    WB analysis of Daudi using 26949-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 26949-1-AP (FCRLA Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FCRLA Polyclonal antibody (26949-1-AP)

    IHC staining of human tonsillitis using 26949-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26949-1-AP (FCRLA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FCRLA Polyclonal antibody (26949-1-AP)

    IHC staining of human tonsillitis using 26949-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26949-1-AP (FCRLA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inRaji cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26949-1-AP is FCRLA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FCRLA fusion protein Ag25331
Full Name Fc receptor-like A
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-43 kDa
GenBank Accession NumberBC006521
Gene Symbol FCRLA
Gene ID (NCBI) 84824
RRIDAB_2880697
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FCRLA antibody 26949-1-APDownload protocol
IHC protocol for FCRLA antibody 26949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols