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FDXR Polyclonal antibody

FDXR Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15584-1-AP

Synonyms

Adrenodoxin reductase, ADXR, Aerredoxin Reductase, AR, FDXR, Ferredoxin NADP(+) reductase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FDXR Polyclonal antibody (15584-1-AP)

    WB analysis using 15584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig adrenal gland tissue using FDXR Polyclonal antibody (15584-1-AP)

    WB analysis of pig adrenal gland using 15584-1-AP

    pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human adrenal gland tissue using FDXR Polyclonal antibody (15584-1-AP)

    WB analysis of human adrenal gland using 15584-1-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FDXR Polyclonal antibody (15584-1-AP)

    IHC staining of human liver cancer using 15584-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15584-1-AP (FDXR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FDXR Polyclonal antibody (15584-1-AP)

    IHC staining of human liver cancer using 15584-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15584-1-AP (FDXR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, pig adrenal gland tissue, human adrenal gland tissue, rat testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15584-1-AP targets FDXR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FDXR fusion protein Ag7145
Full Name ferredoxin reductase
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 48-58 kDa
GenBank Accession NumberBC063493
Gene Symbol FDXR
Gene ID (NCBI) 2232
RRIDAB_2102602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ferredoxin reductase (FDXR, also known as adrenodoxin reductase) is a mitochondrial membrane-associated flavoprotein. One of its functions is to transfer electrons from NADPH to the two human ferredoxin proteins, FDX1 and FDX2. FDXR is expressed in all tissues with the highest expression in tissues such as the adrenal cortex, that specialize in steroid hormone synthesis. Functionally, FDXR is suggested to be involved in various Functionally, FDXR is suggested to be involved in various. Notably, recent studies have shown that FDXR mutations are associated with mitochondrial disorders, probably due to its role in iron-sulfur cluster protein biosynthesis (PMID: 32304229). FDXR has 7 isoforms with the molecular weight of 48-58 kDa.

Protocols

Product Specific Protocols
WB protocol for FDXR antibody 15584-1-APDownload protocol
IHC protocol for FDXR antibody 15584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

The neurosteroid pregnenolone is synthesized by a mitochondrial P450 enzyme other than CYP11A1 in human glial cells.

Authors - Yiqi Christina Lin
View Article
humanWB

Front Oncol

FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation

Authors - Chaojun Yan
  • KD Validated
View Article