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FGFBP2 Polyclonal antibody

FGFBP2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 13254-1-AP

Synonyms

FGF binding protein 2, FGF BP2, FGFBP 2, FGFBP2, HBp17 related protein, HBp17 RP, HBP17RP, KSP37

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using FGFBP2 Polyclonal antibody (13254-1-AP)

    WB analysis of SH-SY5Y using 13254-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13254-1-AP (FGFBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using FGFBP2 Polyclonal antibody (13254-1-AP)

    WB analysis of BxPC-3 using 13254-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13254-1-AP (FGFBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using FGFBP2 Polyclonal antibody (13254-1-AP)

    IF Staining of BxPC-3 using 13254-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 13254-1-AP (FGFBP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells, BxPC-3 cells
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13254-1-AP targets FGFBP2 in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGFBP2 fusion protein Ag4036
Full Name fibroblast growth factor binding protein 2
Calculated Molecular Weight 223 aa, 28 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC025720
Gene Symbol FGFBP2
Gene ID (NCBI) 83888
RRIDAB_2103095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor-binding protein 2 (FGFBP2), also known as 37 kDa killer-specific secretory protein (KSP37), is a member of the fibroblast growth factor binding protein family. FGFBP2 was identified as a Th1/Tc1 specific secretory protein is expressed preferentially in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells and might be involved in essential processes of CTL-mediated immunity. An increase expression of FGFBP2 may be associated with atopic asthma and mild extrinsic asthma. Recently, an observation revealed that FGFBP2 correlate strongly with histology, stage and outcome in ovarian, and it might be a potential prognostic biomarker. The observed MW of FGFBP2 (37 kDa) varies from the predicated MW of 28 kDa, which may be due to the specific space conformation caused by internal disulfide bonds.

Protocols

Product Specific Protocols
WB protocol for FGFBP2 antibody 13254-1-APDownload protocol
IF protocol for FGFBP2 antibody 13254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols