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FGL2 Monoclonal antibody

FGL2 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C9C5

Cat no : 67152-1-Ig

Synonyms

FGL2, FGL-2, fibrinogen like 2, Fibrinogen like protein 2, Fibroleukin, pT49, T49

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using FGL2 Monoclonal antibody (67152-1-Ig)

    WB analysis of THP-1 using 67152-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67152-1-Ig (FGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FGL2 Monoclonal antibody (67152-1-Ig)

    WB analysis of Jurkat using 67152-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67152-1-Ig (FGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using FGL2 Monoclonal antibody (67152-1-Ig)

    IF Staining of mouse spleen using 67152-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using FGL2 antibody (67152-1-Ig, Clone: 2C9C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using FGL2 Monoclonal antibody (67152-1-Ig)

    IF Staining of mouse spleen using 67152-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using FGL2 antibody (67152-1-Ig, Clone: 2C9C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inTHP-1 cells, Jurkat cells
Positive IF detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67152-1-Ig targets FGL2 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FGL2 fusion protein Ag28619
Full Name fibrinogen-like 2
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC017813
Gene Symbol FGL2
Gene ID (NCBI) 10875
RRIDAB_2882449
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation. The protein may play a role in physiologic functions at mucosal sites.

Protocols

Product Specific Protocols
WB protocol for FGL2 antibody 67152-1-IgDownload protocol
IF protocol for FGL2 antibody 67152-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

Targeting fibrinogen-like protein 1 enhances immunotherapy in hepatocellular carcinoma

Authors - Mingen Lin
View Article
mouseWB

Cell Rep

FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation

Authors - Tongguan Tian
View Article