FIS1 Monoclonal antibody
FIS1 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (5)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2F10E1
Cat no : 66635-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, LNCaP cells, HeLa cells, MCF-7 cells, Jurkat cells, HepG2 cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
66635-1-Ig targets FIS1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, rat, goat, fish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FIS1 fusion protein Ag1409 |
| Full Name | fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC009428 |
| Gene Symbol | FIS1 |
| Gene ID (NCBI) | 51024 |
| RRID | AB_2881994 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FIS1 antibody 66635-1-Ig | Download protocol |
| IHC protocol for FIS1 antibody 66635-1-Ig | Download protocol |
| IF protocol for FIS1 antibody 66635-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Pineal Res Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics | ||
Front Immunol Metformin Modulates T Cell Function and Alleviates Liver Injury Through Bioenergetic Regulation in Viral Hepatitis. | ||
J Physiol Sci Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart | ||
Biol Pharm Bull Ampelopsin inhibits breast cancer cell growth through mitochondrial apoptosis pathway | ||
J Mol Histol Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats | ||
Aging (Albany NY) Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Chun (Verified Customer) (06-19-2022) | This antibody worked very well.
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