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Frizzled 9 Polyclonal antibody

Frizzled 9 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13865-1-AP

Synonyms

CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis using 13865-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of mouse testis using 13865-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of HeLa using 13865-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of mouse brain using 13865-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of mouse eye using 13865-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of HUVEC using 13865-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of mouse testis using 13865-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of mouse skeletal muscle using 13865-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    IHC staining of human gliomas using 13865-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13865-1-AP (Frizzled 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    IHC staining of human gliomas using 13865-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13865-1-AP (Frizzled 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, mouse testis tissue, mouse brain tissue, mouse eye tissue, HUVEC cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13865-1-AP targets Frizzled 9 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Frizzled 9 fusion protein Ag4847
Full Name frizzled homolog 9 (Drosophila)
Calculated Molecular Weight 591 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC026333
Gene Symbol FZD9
Gene ID (NCBI) 8326
RRIDAB_2107358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Frizzled 9 antibody 13865-1-APDownload protocol
IHC protocol for Frizzled 9 antibody 13865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

YTHDF1 Regulates Tumorigenicity and Cancer Stem Cell-Like Activity in Human Colorectal Carcinoma.

Authors - Yang Bai
View Article
humanWB

Theranostics

Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.

Authors - Wenjia Liu
View Article