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Frizzled 9 Monoclonal antibody

Frizzled 9 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G9G4

Cat no : 67023-1-Ig

Synonyms

CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Frizzled 9 Monoclonal antibody (67023-1-Ig)

    WB analysis of A549 using 67023-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using Frizzled 9 Monoclonal antibody (67023-1-Ig)

    WB analysis of NCI-H1299 using 67023-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using Frizzled 9 Monoclonal antibody (67023-1-Ig)

    WB analysis of fetal human brain using 67023-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Frizzled 9 Monoclonal antibody (67023-1-Ig)

    WB analysis of HeLa using 67023-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using Frizzled 9 Monoclonal antibody (67023-1-Ig)

    WB analysis of HUVEC using 67023-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Frizzled 9 Monoclonal antibody (67023-1-Ig)

    IHC staining of human gliomas using 67023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Frizzled 9 Monoclonal antibody (67023-1-Ig)

    IHC staining of human gliomas using 67023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Frizzled 9 Monoclonal antibody (67023-1-Ig)

    IF Staining of HEK-293 using 67023-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using Frizzled 9 antibody (67023-1-Ig, Clone: 1G9G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HUVEC cells, NCI-H1299 cells, fetal human brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67023-1-Ig targets Frizzled 9 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Frizzled 9 fusion protein Ag4971
Full Name frizzled homolog 9 (Drosophila)
Calculated Molecular Weight 591 aa, 64 kDa
Observed Molecular Weight 64-70 kDa
GenBank Accession NumberBC026333
Gene Symbol FZD9
Gene ID (NCBI) 8326
RRIDAB_2882338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Frizzled 9 antibody 67023-1-IgDownload protocol
IHC protocol for Frizzled 9 antibody 67023-1-IgDownload protocol
IF protocol for Frizzled 9 antibody 67023-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo

Authors - Jasjot Singh
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Susanne (Verified Customer) (04-17-2023)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Fibroblasts
Frizzled 9 Antibody Immunofluorescence validation (1:500 dilution) in Fibroblasts (Cat no:67023-1-Ig)