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GABPB2 Polyclonal antibody

GABPB2 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20870-1-AP

Synonyms

GABP subunit beta 2, GABPB 2, GABPB2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GABPB2 Polyclonal antibody (20870-1-AP)

    WB analysis of Jurkat using 20870-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GABPB2 Polyclonal antibody (20870-1-AP)

    WB analysis of HeLa using 20870-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GABPB2 Polyclonal antibody (20870-1-AP)

    WB analysis of HEK-293 using 20870-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GABPB2 Polyclonal antibody (20870-1-AP)

    WB analysis of HeLa using 20870-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using GABPB2 Polyclonal antibody (20870-1-AP)

    WB analysis of A375 using 20870-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, A375 cells, HEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20870-1-AP is GABPB2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABPB2 fusion protein Ag14933
Full Name GA binding protein transcription factor, beta subunit 2
Calculated Molecular Weight 448 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC027033
Gene Symbol GABPB2
Gene ID (NCBI) 126626
RRIDAB_10734321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GABPB2 antibody 20870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Mol Cell

Argininosuccinate lyase drives activation of mutant TERT promoter in glioblastomas

Authors - Zhumei Shi
  • KD Validated
View Article