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  • Featured Product
  • KD/KO Validated

GALNT14 Polyclonal antibody

GALNT14 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16939-1-AP

Synonyms

GalNac T10, GalNac T14, GALNT14, GALNT15, pp GaNTase 14

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using GALNT14 Polyclonal antibody (16939-1-AP)

    WB analysis of A549 using 16939-1-AP

    WB result of GALNT14 antibody (16939-1-AP; 1:1200; room temperature for 1.5 hours) with wild-type and GALNT14 knockout A549 cells.

  • Western Blot (WB) analysis of mouse testis tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    WB analysis of mouse testis using 16939-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    WB analysis of mouse kidney using 16939-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    WB analysis of mouse lung using 16939-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    WB analysis of rat testis using 16939-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 Antibody) at dilution of 1:400 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human kidney using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human kidney using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human testis using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human testis using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human skin using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human skin using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human liver using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human liver using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human spleen using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human spleen using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human ovary using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human ovary using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human heart using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GALNT14 Polyclonal antibody (16939-1-AP)

    IHC staining of human heart using 16939-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, mouse testis tissue, mouse kidney tissue, mouse lung tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16939-1-AP targets GALNT14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALNT14 fusion protein Ag10470
Full Name UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 14 (GalNAc-T14)
Calculated Molecular Weight 552 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC010659
Gene Symbol GALNT14
Gene ID (NCBI) 79623
RRIDAB_2232200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GALNT14 antibody 16939-1-APDownload protocol
IHC protocol for GALNT14 antibody 16939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

GalNAc-T14 promotes metastasis through Wnt dependent HOXB9 expression in lung adenocarcinoma.

Authors - Ok-Seon Kwon
  • KD Validated
View Article

Mol Cancer Res

BORIS expression in ovarian cancer precursor cells alters the CTCF cistrome and enhances invasiveness through GALNT14.

Authors - Joanna C Hillman
View Article
humanIHC

Front Immunol

Vessel state and immune infiltration of the angiogenesis subgroup and construction of a prediction model in osteosarcoma

Authors - Jintao Wu
View Article
humanWB

Front Immunol

Molecular characterization of immunogenic cell death indicates prognosis and tumor microenvironment infiltration in osteosarcoma

Authors - Zhongyue Liu
  • KD Validated
View Article