GART Polyclonal antibody

GART Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13659-1-AP

Synonyms

AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, PAIS, PGFT, PRGS



Tested Applications

Positive WB detected inA431 cells, HepG2 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13659-1-AP targets GART in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GART fusion protein Ag4555
Full Name phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
Calculated Molecular Weight 1010 aa, 108 kDa
Observed Molecular Weight 108 kDa, 46 kDa
GenBank Accession NumberBC038958
Gene Symbol GART
Gene ID (NCBI) 2618
RRIDAB_2107937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GART is a trifunctional polypeptide which has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing.

Protocols

Product Specific Protocols
WB protocol for GART antibody 13659-1-APDownload protocol
IHC protocol for GART antibody 13659-1-APDownload protocol
IF protocol for GART antibody 13659-1-APDownload protocol
IP protocol for GART antibody 13659-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Science

mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.

Authors - Issam Ben-Sahra
humanWB

Mol Cell

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

Authors - Eunus S Ali
mouseWB

Nat Commun

Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.

Authors - Mona Hoseini Soflaee
humanWB

EMBO J

UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.

Authors - Xing Feng
humanWB

Cell Rep

Purine Nucleotide Availability Regulates mTORC1 Activity through the Rheb GTPase.

Authors - Natasha Emmanuel
humanWB

Nat Metab

Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.

Authors - Diem H Tran

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (08-26-2019)

Works well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HepG2, mouse liver, mouse lung, mouse hippocampus
GART Antibody Western Blot, validation (1:500 dilution) in HepG2, mouse liver, mouse lung, mouse hippocampus (Cat no:13659-1-AP)