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GATAD2B Polyclonal antibody

GATAD2B Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF

Conjugate

Unconjugated

Cat no : 25679-1-AP

Synonyms

GATAD2B, KIAA1150, p66/p68, P66beta, RP11 216N14.6

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of A549 using 25679-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of PC-3 using 25679-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of HEK-293 using 25679-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of K-562 using 25679-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of MCF-7 using 25679-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of HeLa using 25679-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    IF Staining of A549 using 25679-1-AP

    Immunofluorescent analysis of A549 cells using 25679-1-AP (GATAD2B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, PC-3 cells
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25679-1-AP is GATAD2B Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GATAD2B fusion protein Ag22615
Full Name GATA zinc finger domain containing 2B
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC069419
Gene Symbol GATAD2B
Gene ID (NCBI) 57459
RRIDAB_2880190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GATAD2B antibody 25679-1-APDownload protocol
IF protocol for GATAD2B antibody 25679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila
View Article