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  • Featured Product
  • KD/KO Validated

GBP2 Polyclonal antibody

GBP2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11854-1-AP

Synonyms

GBP 2, GBP2, GTP binding protein 2, HuGBP 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis using 11854-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis using 11854-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis of human placenta using 11854-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis of K-562 using 11854-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis of human lung using 11854-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human spleen using 11854-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human spleen using 11854-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human tonsillitis using 11854-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human tonsillitis using 11854-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GBP2 Polyclonal antibody (11854-1-AP)

    IF Staining of HeLa using 11854-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBP2 antibody (11854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse spleen tissue, human placenta tissue, A375 cells, K-562 cells, human lung tissue, rat spleen tissue
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11854-1-AP targets GBP2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, Toxoplasma
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP2 fusion protein Ag2425
Full Name guanylate binding protein 2, interferon-inducible
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC022272
Gene Symbol GBP2
Gene ID (NCBI) 2634
RRIDAB_2109336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness, because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

Protocols

Product Specific Protocols
WB protocol for GBP2 antibody 11854-1-APDownload protocol
IHC protocol for GBP2 antibody 11854-1-APDownload protocol
IF protocol for GBP2 antibody 11854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nature

Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.

Authors - Peng Li
View Article
mouseWB,IHC,IF

Nature

Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.

Authors - Etienne Meunier
View Article
mouseWB

Cell

IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.

Authors - Rajendra Karki
View Article
humanWB

Cell

IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.

Authors - Si Ming Man
View Article
mouseWB

Nat Immunol

The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.

Authors - Si Ming Man
View Article
mouseWB,IF

Nat Immunol

Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.

Authors - Etienne Meunier
  • KO Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Kyosuke (Verified Customer) (06-12-2019)

This antibody works very well for WB using HUVECs.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HUVECs