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GEMIN6 Polyclonal antibody

GEMIN6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12307-2-AP

Synonyms

Gem associated protein 6, Gemin 6, GEMIN6, SIP2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GEMIN6 Polyclonal antibody (12307-2-AP)

    WB analysis of HEK-293 using 12307-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12307-2-AP (GEMIN6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GEMIN6 Polyclonal antibody (12307-2-AP)

    WB analysis of HeLa using 12307-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12307-2-AP (GEMIN6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GEMIN6 Polyclonal antibody (12307-2-AP)

    WB analysis of HEK-293 using 12307-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12307-2-AP (GEMIN6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GEMIN6 Polyclonal antibody (12307-2-AP)

    IHC staining of human breast cancer using 12307-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12307-2-AP (GEMIN6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GEMIN6 Polyclonal antibody (12307-2-AP)

    IHC staining of human breast cancer using 12307-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12307-2-AP (GEMIN6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using GEMIN6 Polyclonal antibody (12307-2-AP)

    IF Staining of PC-3 using 12307-2-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using 12307-2-AP (GEMIN6 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12307-2-AP targets GEMIN6 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GEMIN6 fusion protein Ag2954
Full Name gem (nuclear organelle) associated protein 6
Calculated Molecular Weight 167 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC018195
Gene Symbol GEMIN6
Gene ID (NCBI) 79833
RRIDAB_2111835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GEMIN6, also named as SIP2, is a subunit of SMN complex. It plays an important role in the splicing of cellular pre-mRNAs.

Protocols

Product Specific Protocols
WB protocol for GEMIN6 antibody 12307-2-APDownload protocol
IHC protocol for GEMIN6 antibody 12307-2-APDownload protocol
IF protocol for GEMIN6 antibody 12307-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Authors - Anne-Sophie Gribling-Burrer
View Article
humanWB

Nucleic Acids Res

NUFIP and the HSP90/R2TP chaperone bind the SMN complex and facilitate assembly of U4-specific proteins.

Authors - Jonathan Bizarro
View Article