GGCT Polyclonal antibody

GGCT Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16257-1-AP

Synonyms

C7orf24, CRF21, Gamma glutamylcyclotransferase, GCTG, Ggc, GGCT



Tested Applications

Positive WB detected inHeLa cells, U-937 cells, mouse kidney tissue, mouse liver tissue, human brain tissue, HepG2 cells, HEK-293 cells, Jurkat cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16257-1-AP targets GGCT in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GGCT fusion protein Ag9284
Full Name gamma-glutamyl cyclotransferase
Calculated Molecular Weight 188 aa, 21 kDa
Observed Molecular Weight 21-25 kDa
GenBank Accession NumberBC019243
Gene Symbol GGCT
Gene ID (NCBI) 79017
RRIDAB_10852962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GGCT antibody 16257-1-APDownload protocol
IHC protocol for GGCT antibody 16257-1-APDownload protocol
IF protocol for GGCT antibody 16257-1-APDownload protocol
IP protocol for GGCT antibody 16257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.

Authors - Kenneth N Maclean
humanWB

Sci Rep

Identification of proteins responsible for adriamycin resistance in breast cancer cells using proteomics analysis.

Authors - Zhipeng Wang
mouseWB

Biochim Biophys Acta Mol Basis Dis

Integrated application of transcriptome and metabolomics reveals potential therapeutic targets for the polarization of atherosclerotic macrophages

Authors - Lin Shen
humanWB,IHC,CoIP

Int J Mol Sci

Interaction of MRPL9 and GGCT Promotes Cell Proliferation and Migration by Activating the MAPK/ERK Pathway in Papillary Thyroid Cancer

Authors - Hui-Min Zhang
mouseWB,IHC

Redox Biol

Unbalanced redox status network as an early pathological event in congenital cataracts

Authors - Eloy Bejarano