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GLDN Polyclonal antibody

GLDN Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26185-1-AP

Synonyms

CLOM, COLM, CRG L2, CRGL2, GLDN, gliomedin, UNC 112

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using GLDN Polyclonal antibody (26185-1-AP)

    WB analysis of human placenta using 26185-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 26185-1-AP (GLDN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using GLDN Polyclonal antibody (26185-1-AP)

    IHC staining of human brain using 26185-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26185-1-AP (GLDN Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using GLDN Polyclonal antibody (26185-1-AP)

    IHC staining of human brain using 26185-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26185-1-AP (GLDN Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 26185-1-AP is GLDN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLDN fusion protein Ag24247
Full Name gliomedin
Calculated Molecular Weight 551 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC113399
Gene Symbol GLDN
Gene ID (NCBI) 342035
RRIDAB_2880416
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GLDN antibody 26185-1-APDownload protocol
IHC protocol for GLDN antibody 26185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Genes (Basel)

Genome-Wide Association Study Adjusted for Occupational and Environmental Factors for Bladder Cancer Susceptibility.

Authors - Takumi Takeuchi
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Hongxue (Verified Customer) (05-13-2022)

WB with non-specific band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse liver
GLDN Antibody Western Blot validation (1:1000 dilution) in Mouse liver (Cat no:26185-1-AP)