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GLE1 Polyclonal antibody

GLE1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 26466-1-AP

Synonyms

GLE1, GLE1 like protein, GLE1L, hGLE1, LCCS, LCCS1, Nucleoporin GLE1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLE1 Polyclonal antibody (26466-1-AP)

    WB analysis using 26466-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26466-1-AP (GLE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using GLE1 Polyclonal antibody (26466-1-AP)

    WB analysis of A549 using 26466-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26466-1-AP (GLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GLE1 Polyclonal antibody (26466-1-AP)

    WB analysis of MCF-7 using 26466-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26466-1-AP (GLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GLE1 Polyclonal antibody (26466-1-AP)

    WB analysis of PC-3 using 26466-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26466-1-AP (GLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GLE1 Polyclonal antibody (26466-1-AP)

    IF Staining of HepG2 using 26466-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26466-1-AP (GLE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells, PC-3 cells, HepG2 cells, Jurkat cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26466-1-AP targets GLE1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLE1 fusion protein Ag24208
Full Name GLE1 RNA export mediator homolog (yeast)
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 79-80 kDa
GenBank Accession NumberBC030012
Gene Symbol GLE1
Gene ID (NCBI) 2733
RRIDAB_2880525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GLE1 antibody 26466-1-APDownload protocol
IF protocol for GLE1 antibody 26466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols