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ZGLP1 Polyclonal antibody

ZGLP1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55292-1-AP

Synonyms

GATA like protein 1, GLP 1, GLP1, GLP-1, ZGLP1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using ZGLP1 Polyclonal antibody (55292-1-AP)

    WB analysis of mouse ovary using 55292-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 55292-1-AP (ZGLP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using ZGLP1 Polyclonal antibody (55292-1-AP)

    IHC staining of human ovary using 55292-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 55292-1-AP (GLP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ZGLP1 Polyclonal antibody (55292-1-AP)

    IHC staining of human ovary using 55292-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 55292-1-AP (GLP1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse ovary tissue
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55292-1-AP targets ZGLP1 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GATA like protein-1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberNM_001103167
Gene Symbol GLP-1
Gene ID (NCBI) 100125288
RRIDAB_2881301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZGLP1, also known as GLP-1, is a nuclear protein containing two zinc fingers, one of which is highly homologous to the conserved GATA zinc finger DNA binding motif. It is a transcriptional repressor that plays a central role in somatic cells of the gonad and is required for germ cell development.

Protocols

Product Specific Protocols
WB protocol for ZGLP1 antibody 55292-1-APDownload protocol
IHC protocol for ZGLP1 antibody 55292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols