GNAI2 Polyclonal antibody
GNAI2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11136-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, SKOV-3 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue, human kidney tissue, human lung cancer tissue, mouse ovary tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
11136-1-AP targets GNAI2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GNAI2 fusion protein Ag1597 |
| Full Name | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC012138 |
| Gene Symbol | GNAI2 |
| Gene ID (NCBI) | 2771 |
| RRID | AB_2232442 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNAI2 antibody 11136-1-AP | Download protocol |
| IHC protocol for GNAI2 antibody 11136-1-AP | Download protocol |
| IF protocol for GNAI2 antibody 11136-1-AP | Download protocol |
| IP protocol for GNAI2 antibody 11136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
bioRxiv Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis | ||
Adv Sci (Weinh) Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC | ||
Sci Adv RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells |
