- Featured Product
- KD/KO Validated
GNPAT Polyclonal antibody
GNPAT Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14931-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, human placenta tissue, Transfected HEK-293 cells, COLO 320 cells, HeLa cells, PC-3 cells, mouse brain tissue |
| Positive IP detected in | COLO 320 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
Product Information
14931-1-AP targets GNPAT in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GNPAT fusion protein Ag6735 |
| Full Name | glyceronephosphate O-acyltransferase |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 65-69 kDa |
| GenBank Accession Number | BC000450 |
| Gene Symbol | GNPAT |
| Gene ID (NCBI) | 8443 |
| RRID | AB_2110546 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID:21990100) This antibody recognize the 69 kDa GNPAT protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNPAT antibody 14931-1-AP | Download protocol |
| IP protocol for GNPAT antibody 14931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Host Microbe Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.
| ||
J Clin Invest Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission. | ||
Int J Mol Sci Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors. | ||
Hum Mutat Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3. | ||
Front Cell Dev Biol Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.
| ||
Histochem Cell Biol C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism. |
