gephyrin Polyclonal antibody

gephyrin Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12681-1-AP

Synonyms

Domain E, GEPH, gephyrin, GPH, GPHN, GPHRYN, KIAA1385, MPT adenylyltransferase, MPT Mo transferase



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12681-1-AP targets gephyrin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen gephyrin fusion protein Ag3370
Full Name gephyrin
Calculated Molecular Weight 769 aa, 83 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC030016
Gene Symbol GPHN
Gene ID (NCBI) 10243
RRIDAB_2247770
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.

Protocols

Product Specific Protocols
WB protocol for gephyrin antibody 12681-1-APDownload protocol
IHC protocol for gephyrin antibody 12681-1-APDownload protocol
IF protocol for gephyrin antibody 12681-1-APDownload protocol
IP protocol for gephyrin antibody 12681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neurosci

Microglial Tmem59 Deficiency Impairs Phagocytosis of Synapse and Leads to Autism-Like Behaviors in Mice.

Authors - Jian Meng
mouseWB

Cell Rep

Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.

Authors - Giuseppe Sciamanna
mouseWB

Neurosci Lett

Microglia participate in postoperative cognitive dysfunction by mediating the loss of inhibitory synapse through the complement pathway

Authors - Xiaoxiang Tan
mouseWB

Synapse

Triptolide injection reduces Alzheimer's disease-like pathology in mice

Authors - Rui Mao
mouseIF

Arthritis Rheumatol

Anti-Gephyrin antibodies: A novel specificity in patients with systemic sclerosis and lower bowel dysfunction

Authors - Zsuzsanna H McMahan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Reyes (Verified Customer) (03-01-2024)

Gephyrin antibody (in red) works on human brain paraffin sections, although being found in the synapsis would make it difficult to quantify in the tissue.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
gephyrin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:12681-1-AP)
FH

Deria (Verified Customer) (07-14-2022)

This antibody works fine

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: brain tissue