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GPR105 Polyclonal antibody

GPR105 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 20190-1-AP

Synonyms

G protein coupled receptor 105, GPR105, KIAA0001, P2RY14, P2Y purinoceptor 14, P2Y14, UDP glucose receptor

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human stomach tissue using GPR105 Polyclonal antibody (20190-1-AP)

    IHC staining of human stomach using 20190-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20190-1-AP (GPR105 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using GPR105 Polyclonal antibody (20190-1-AP)

    IHC staining of human stomach using 20190-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20190-1-AP (GPR105 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using GPR105 Polyclonal antibody (20190-1-AP)

    IHC staining of human placenta using 20190-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20190-1-AP (GPR105 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using GPR105 Polyclonal antibody (20190-1-AP)

    IHC staining of human placenta using 20190-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20190-1-AP (GPR105 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman stomach tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

20190-1-AP targets GPR105 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR105 fusion protein Ag14105
Full Name purinergic receptor P2Y, G-protein coupled, 14
Calculated Molecular Weight 338 aa, 39 kDa
GenBank Accession NumberBC034989
Gene Symbol P2RY14
Gene ID (NCBI) 9934
RRIDAB_10693612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR105 (also known as P2RY14) is widely expressed throughout many brain regions and peripheral tissues of humans and rodents, and couples to a pertussis toxin-sensitive G protein (PMID: 14559350). Notably, GPR105 is also prominently expressed in immune cells, including macrophages, lymphocytes, and neutrophils (PMID: 22778393).

Protocols

Product Specific Protocols
IHC protocol for GPR105 antibody 20190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Mol Brain

Systematic analysis of expression signatures of neuronal subpopulations in the VTA.

Authors - Hyun Jin Kim
View Article