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GPR17 Polyclonal antibody

GPR17 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC,ELISA

Conjugate

Unconjugated

Cat no : 13416-1-AP

Synonyms

G protein coupled receptor 17, GPR17, P2Y like receptor, R12, UDP/CysLT receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using GPR17 Polyclonal antibody (13416-1-AP)

    WB analysis of human plasma using 13416-1-AP

    human serum were subjected to SDS PAGE followed by western blot with 13416-1-AP (GPR17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using GPR17 Polyclonal antibody (13416-1-AP)

    FC experiment of SH-SY5Y using 13416-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug GPR17 antibody (13416-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inhuman serum tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13416-1-AP targets GPR17 in WB, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR17 fusion protein Ag4106
Full Name G protein-coupled receptor 17
Calculated Molecular Weight 367 aa, 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC031653
Gene Symbol GPR17
Gene ID (NCBI) 2840
RRIDAB_2279341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GPR17 antibody 13416-1-APDownload protocol
FC protocol for GPR17 antibody 13416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols