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GPRASP1-Specific Polyclonal antibody

GPRASP1-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,IHC,ELISA

Conjugate

Unconjugated

Cat no : 20020-1-AP

Synonyms

GASP, GASP 1, GASP1, GPRASP1, GPRASP1-Specific, KIAA0443

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    WB analysis of HeLa using 20020-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    WB analysis of human brain using 20020-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    WB analysis of HepG2 using 20020-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    WB analysis of mouse brain using 20020-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    IHC staining of human brain using 20020-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    IHC staining of human brain using 20020-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20020-1-AP targets GPRASP1-Specific in WB,IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name G protein-coupled receptor associated sorting protein 1
Calculated Molecular Weight 157 kDa
Observed Molecular Weight 157 kDa
GenBank Accession NumberNM_014710
Gene Symbol GPRASP1
Gene ID (NCBI) 9737
RRIDAB_10694144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPRASP1, also named as GASP, GASP1 and KIAA0443, belongs to the GPRASP family. GPRASP1 may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It is targets receptors for degradation in lysosomes. The antibody is specific to GPRASP1.

Protocols

Product Specific Protocols
WB protocol for GPRASP1-Specific antibody 20020-1-APDownload protocol
IHC protocol for GPRASP1-Specific antibody 20020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Aging (Albany NY)

Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation

Authors - Baining Zhang
View Article