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CSN1 Polyclonal antibody

CSN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11709-1-AP

Synonyms

COPS1, CSN1, G protein pathway suppressor 1, GPS 1, GPS1, Protein MFH, SGN1, Signalosome subunit 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSN1 Polyclonal antibody (11709-1-AP)

    WB analysis using 11709-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CSN1 Polyclonal antibody (11709-1-AP)

    WB analysis of MCF-7 using 11709-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CSN1 Polyclonal antibody (11709-1-AP)

    WB analysis of K-562 using 11709-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CSN1 Polyclonal antibody (11709-1-AP)

    WB analysis of HEK-293 using 11709-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using CSN1 Polyclonal antibody (11709-1-AP)

    WB analysis of HT-1080 using 11709-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CSN1 Polyclonal antibody (11709-1-AP)

    WB analysis of Raji using 11709-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CSN1 Polyclonal antibody (11709-1-AP)

    IP experiment of HeLa using 11709-1-AP

    IP result of anti-CSN1 (IP:11709-1-AP, 4ug; Detection:11709-1-AP 1:500) with HeLa cells lysate 2600 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CSN1 Polyclonal antibody (11709-1-AP)

    IHC staining of human prostate cancer using 11709-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11709-1-AP (CSN1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CSN1 Polyclonal antibody (11709-1-AP)

    IF Staining of MCF-7 using 11709-1-AP

    Immunofluorescent analysis of MCF-7 cells using 11709-1-AP (CSN1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HT-1080 cells, Raji cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11709-1-AP targets CSN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSN1 fusion protein Ag2273
Full Name G protein pathway suppressor 1
Calculated Molecular Weight 491 aa, 55-60 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC000155
Gene Symbol GPS1
Gene ID (NCBI) 2873
RRIDAB_2232685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CSN1 antibody 11709-1-APDownload protocol
IHC protocol for CSN1 antibody 11709-1-APDownload protocol
IF protocol for CSN1 antibody 11709-1-APDownload protocol
IP protocol for CSN1 antibody 11709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

CSN1 facilitates proliferation and migration of hepatocellular carcinoma cells by upregulating cyclin A2 expression.

Authors - Hangwei Fu
View Article