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GRAP Polyclonal antibody

GRAP Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14505-1-AP

Synonyms

GRAP, GRB2 related adapter protein, GRB2 related adaptor protein

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using GRAP Polyclonal antibody (14505-1-AP)

    WB analysis of U-937 using 14505-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 14505-1-AP (GRAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using GRAP Polyclonal antibody (14505-1-AP)

    IHC staining of human lymphoma using 14505-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 14505-1-AP (GRAP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using GRAP Polyclonal antibody (14505-1-AP)

    IHC staining of human lymphoma using 14505-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 14505-1-AP (GRAP antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14505-1-AP targets GRAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRAP fusion protein Ag5962
Full Name GRB2-related adaptor protein
Calculated Molecular Weight 25 kDa
GenBank Accession NumberBC063035
Gene Symbol GRAP
Gene ID (NCBI) 10750
RRIDAB_2112704
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GRAP antibody 14505-1-APDownload protocol
IHC protocol for GRAP antibody 14505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Life Sci

Grb2-related adaptor protein GRAP is a novel regulator of liver fibrosis

Authors - Xiaoyan Wu
View Article