GRASP65,GORASP1 Monoclonal antibody
GRASP65,GORASP1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1B2C10
Cat no : 66651-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HSC-T6 cells, HEK-293 cells, MCF-7 cells, Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
66651-1-Ig targets GRASP65,GORASP1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GRASP65,GORASP1 fusion protein Ag1054 |
| Full Name | golgi reassembly stacking protein 1, 65kDa |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC008928 |
| Gene Symbol | GORASP1 |
| Gene ID (NCBI) | 64689 |
| RRID | AB_2882008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRASP65,GORASP1 antibody 66651-1-Ig | Download protocol |
| IHC protocol for GRASP65,GORASP1 antibody 66651-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration. | ||
Mol Biol Cell Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking. | ||
J Cell Biochem Golgi apparatus fragmentation participates in oxidized low-density lipoprotein-induced endothelial cell injury. |
