• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

mGluR1 Polyclonal antibody

mGluR1 Polyclonal Antibody for FC, IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19955-1-AP

Synonyms

GPRC1A, GRM1, GRM1A, mGlu1, mGluR1, MGLUR1A

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse brain tissue using mGluR1 Polyclonal antibody (19955-1-AP)

    IHC staining of mouse brain using 19955-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19955-1-AP (mGluR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using mGluR1 Polyclonal antibody (19955-1-AP)

    IHC staining of mouse brain using 19955-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19955-1-AP (mGluR1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using mGluR1 Polyclonal antibody (19955-1-AP)

    IHC staining of mouse brain using 19955-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19955-1-AP (mGluR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using mGluR1 Polyclonal antibody (19955-1-AP)

    IHC staining of mouse brain using 19955-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19955-1-AP (mGluR1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using mGluR1 Polyclonal antibody (19955-1-AP)

    IF Staining of mouse brain using 19955-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 19955-1-AP (mGluR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using mGluR1 Polyclonal antibody (19955-1-AP)

    FC experiment of SH-SY5Y using 19955-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug mGluR1 antibody (19955-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19955-1-AP targets mGluR1 in IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glutamate receptor, metabotropic 1
Calculated Molecular Weight 132 kDa
GenBank Accession NumberNM_000838
Gene Symbol GRM1
Gene ID (NCBI) 2911
RRIDAB_10638312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRM1, also named as GPRC1A and MGLUR1, belongs to the G-protein coupled receptor 3 family. GRM1 is a receptor for glutamate. The activity of GRM1 is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. It may participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. The antibody can recognize isoform1 and isoform2 of GRM1.

Protocols

Product Specific Protocols
IHC protocol for mGluR1 antibody 19955-1-APDownload protocol
IF protocol for mGluR1 antibody 19955-1-APDownload protocol
FC protocol for mGluR1 antibody 19955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Clin Cancer Res

Serum glutamate levels correlate with Gleason score and glutamate blockade decreases proliferation, migration, and invasion and induces apoptosis in prostate cancer cells.

Authors - Koochekpour Shahriar S
View Article
human

Int J Biol Sci

Glutamate signaling in benign and malignant disorders: current status, future perspectives, and therapeutic implications.

Authors - Stacey S Willard
View Article
human

Brain Commun

M1 muscarinic acetylcholine receptor dysfunction in moderate Alzheimer's disease pathology.

Authors - Jee Hyun Yi
View Article