mGluR2 Polyclonal antibody
mGluR2 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
Cat no : 19956-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, HeLa cells, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
19956-1-AP targets mGluR2 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | glutamate receptor, metabotropic 2 |
| Calculated Molecular Weight | 96 kDa |
| Observed Molecular Weight | 96 kDa, 120 kDa |
| GenBank Accession Number | NM_000839 |
| Gene Symbol | GRM2 |
| Gene ID (NCBI) | 2912 |
| RRID | AB_10638447 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GRM2, also named as GPRC1B and MGLUR2, belongs to the G-protein coupled receptor 3 family. GRM2 is a receptor for glutamate. The activity of GRM2 is mediated by a G-protein that inhibits adenylate cyclase activity. It may mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for mGluR2 antibody 19956-1-AP | Download protocol |
| IHC protocol for mGluR2 antibody 19956-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Res Long-range epigenetic silencing associates with deregulation of Ikaros targets in colorectal cancer cells. | ||
Auton Neurosci Expression of group II metabotropic glutamate receptors in rat superior cervical ganglion |
