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- KD/KO Validated
GSTO1 Polyclonal antibody
GSTO1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15124-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, rat brain tissue, mouse liver tissue, human heart tissue, U-937 cells, Jurkat cells, PC-3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
| IF | See 2 publications below |
Product Information
15124-1-AP targets GSTO1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GSTO1 fusion protein Ag7199 |
| Full Name | glutathione S-transferase omega 1 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 27-30 kDa |
| GenBank Accession Number | BC000127 |
| Gene Symbol | GSTO1 |
| Gene ID (NCBI) | 9446 |
| RRID | AB_2248132 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells. Human GSTO1 assembles as a dimer. This antibody detects both monomer (27-30 kDa) and dimer forms of GSTO1 (54-64 kDa). (PMID: 21495794)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GSTO1 antibody 15124-1-AP | Download protocol |
| IHC protocol for GSTO1 antibody 15124-1-AP | Download protocol |
| IF protocol for GSTO1 antibody 15124-1-AP | Download protocol |
| IP protocol for GSTO1 antibody 15124-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation | ||
J Exp Med ASC deglutathionylation is a checkpoint for NLRP3 inflammasome activation.
| ||
Food Chem TMT-based quantitative proteomic analysis of porcine muscle associated with postmortem meat quality. | ||
Biochem J Selenophosphate Synthetase 1 is an Essential Protein with Roles in Regulation of Redox Homeostasis in Mammals. | ||
J Proteomics Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection. |
