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GUCY1B3 Antibody

GUCY1B3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19011-1-AP

Synonyms

GC S beta 1, GC SB3, GCS beta 1, GCS beta 3, GUC1B3, GUCB3, GUCSB3, GUCY1B1, GUCY1B3



Tested Applications

Positive WB detected inhuman placenta tissue, mouse lung tissue, rat lung tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inmouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

19011-1-AP targets GUCY1B3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GUCY1B3 fusion protein Ag5196
Full Name guanylate cyclase 1, soluble, beta 3
Calculated molecular weight 71 kDa
Observed molecular weight 67-71 kDa
GenBank accession number BC047620
Gene symbol GUCY1B3
Gene ID (NCBI) 2983
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GUCY1B3 antibody 19011-1-APDownload protocol
IHC protocol for GUCY1B3 antibody 19011-1-APDownload protocol
IF protocol for GUCY1B3 antibody 19011-1-APDownload protocol
IP protocol for GUCY1B3 antibody 19011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Tissue Res

Cavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscle.

Authors - Baoyi Zhu
mouseWB,IHC

Sci Rep

Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.

Authors - Catarina Rippe
mouseWB,IHC

Clin Sci (Lond)

Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.

Authors - Mei Xue
  • KD Validated