GUCY2F Polyclonal antibody

GUCY2F Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25252-1-AP

Synonyms

CYGF, GC F, guanylate cyclase 2F, retinal, Guanylate cyclase F, GUC2DL, GUC2F, GUCY2F, RETGC 2, RETGC2, Retinal guanylyl cyclase 2, ROS GC2



Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 25252-1-AP is GUCY2F Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GUCY2F fusion protein Ag19266
Full Name guanylate cyclase 2F, retinal
Calculated Molecular Weight 1108 aa, 125 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC156674
Gene Symbol GUCY2F
Gene ID (NCBI) 2986
RRIDAB_2879989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GUCY2F(Guanylate cyclase 2F, retinal) is also named as GUC2F, RETGC2 and belongs to the adenylyl cyclase class-4/guanylyl cyclase family. GUCY2F may play a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. By in situ hybridization, mRNA encoding GUCY2F is found only in the outer nuclear layer and inner segments of photoreceptor cells. By using synthetic peptide antiserum specific for each RetGC subtype, RetGC-2(GUCY2F) can be distinguished from RetGC-1 as a slightly smaller protein in immunoblots of bovine rod outer segments.(PMID:7777544).

Protocols

Product Specific Protocols
WB protocol for GUCY2F antibody 25252-1-APDownload protocol
IHC protocol for GUCY2F antibody 25252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

iScience

A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.

Authors - Suguru Yamasaki