• Featured Product
  • KD/KO Validated

HACE1 Polyclonal antibody

HACE1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24104-1-AP

Synonyms

HACE1, KIAA1320



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24104-1-AP is HACE1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HACE1 fusion protein Ag21130
Full Name HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
Calculated Molecular Weight 909 aa, 102 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC034982
Gene Symbol HACE1
Gene ID (NCBI) 57531
RRIDAB_2879432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HACE1(E3 ubiquitin-protein ligase HACE1) also named as KIAA1320, has a tumor-suppressor function dependent on its E3 ligase activity and controlling cell cycle progression during cell stress through degradation of cyclin D1. It belongs to the HECT family of E3 ubiqutin protein ligase. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 102 kDa, 36 kDa, 64 kDa and 78 kDa.

Protocols

Product Specific Protocols
WB protocol for HACE1 antibody 24104-1-APDownload protocol
IHC protocol for HACE1 antibody 24104-1-APDownload protocol
IF protocol for HACE1 antibody 24104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Reprod Biol Endocrinol

NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes

Authors - Shiwei Wang
  • KD Validated
humanIHC

Cancer Med

Overexpression of HACE1 in gastric cancer inhibits tumor aggressiveness by impeding cell proliferation and migration.

Authors - Ying-Ling Chen
  • KO Validated