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HADH Polyclonal antibody

HADH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19828-1-AP

Synonyms

HAD, HADH, HADH1, HADHSC, HCDH, HHF4, M/SCHAD, SCHAD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HADH Polyclonal antibody (19828-1-AP)

    WB analysis using 19828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HADH Polyclonal antibody (19828-1-AP)

    WB analysis of mouse liver using 19828-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using HADH Polyclonal antibody (19828-1-AP)

    WB analysis of mouse pancreas using 19828-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HADH Polyclonal antibody (19828-1-AP)

    WB analysis of HepG2 using 19828-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using HADH Polyclonal antibody (19828-1-AP)

    WB analysis of mouse skeletal muscle using 19828-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HADH Polyclonal antibody (19828-1-AP)

    WB analysis of Jurkat using 19828-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using HADH Polyclonal antibody (19828-1-AP)

    WB analysis of mouse heart using 19828-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using HADH Polyclonal antibody (19828-1-AP)

    WB analysis of mouse kidney using 19828-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using HADH Polyclonal antibody (19828-1-AP)

    IP experiment of HepG2 using 19828-1-AP

    IP result of anti-HADH (IP:19828-1-AP, 3ug; Detection:19828-1-AP 1:700) with HepG2 cells lysate 400ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HADH Polyclonal antibody (19828-1-AP)

    IHC staining of human breast cancer using 19828-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HADH Polyclonal antibody (19828-1-AP)

    IHC staining of human liver cancer using 19828-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HADH Polyclonal antibody (19828-1-AP)

    IHC staining of human liver cancer using 19828-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using HADH Polyclonal antibody (19828-1-AP)

    IHC staining of human hepatocirrhosis using 19828-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using HADH Polyclonal antibody (19828-1-AP)

    IHC staining of human hepatocirrhosis using 19828-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using HADH Polyclonal antibody (19828-1-AP)

    IHC staining of human hepatocirrhosis using 19828-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using HADH Polyclonal antibody (19828-1-AP)

    IHC staining of human hepatocirrhosis using 19828-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HADH Polyclonal antibody (19828-1-AP)

    IF Staining of HepG2 using 19828-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 19828-1-AP (HADH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19828-1-AP targets HADH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HADH fusion protein Ag13928
Full Name hydroxyacyl-Coenzyme A dehydrogenase
Calculated Molecular Weight 314 aa, 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC000306
Gene Symbol HADH
Gene ID (NCBI) 3033
RRIDAB_10667408
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HADH(Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) is also named as HAD, HADHSC, SCHAD and belongs to the 3-hydroxyacyl-CoA dehydrogenase family. It catalyzes the reversible dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs with concomitant reduction of NAD to NADH and exerts it highest activity toward 3-hydroxydecanoyl-CoA. . Human HADH encodes a deduced 314-amino acid protein composed of a 12-residue mitochondrial import signal peptide and a 302-residue mature HADH protein with a calculated molecular mass of 34.3 kD.

Protocols

Product Specific Protocols
WB protocol for HADH antibody 19828-1-APDownload protocol
IHC protocol for HADH antibody 19828-1-APDownload protocol
IF protocol for HADH antibody 19828-1-APDownload protocol
IP protocol for HADH antibody 19828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Pyruvate-supported flux through medium-chain ketothiolase promotes mitochondrial lipid tolerance in cardiac and skeletal muscles

Authors - Timothy R Koves
View Article
humanIP, WB

Hepatology

Disruption of the histidine triad nucleotide-binding hint2 gene in mice affects glycemic control and mitochondrial function.

Authors - Martin Juliette J
View Article
mouseWB

EMBO Rep

SCFJFK is functionally linked to obesity and metabolic syndrome.

Authors - Lin He
View Article
ratWB

EBioMedicine

PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.

Authors - Zhongshi Lyu
View Article
humanWB

Front Oncol

Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.

Authors - Jinghui Lu
View Article
mouseWB

Sci Rep

Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.

Authors - Lei Wu
View Article