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HAPLN4 Polyclonal antibody

HAPLN4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21228-1-AP

Synonyms

Brain link protein 2, BRAL2, HAPLN4, KIAA1926

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using HAPLN4 Polyclonal antibody (21228-1-AP)

    WB analysis of mouse brain using 21228-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21228-1-AP (HAPLN4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using HAPLN4 Polyclonal antibody (21228-1-AP)

    IHC staining of human brain using 21228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21228-1-AP (HAPLN4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using HAPLN4 Polyclonal antibody (21228-1-AP)

    IHC staining of human brain using 21228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21228-1-AP (HAPLN4 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21228-1-AP is HAPLN4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAPLN4 fusion protein Ag15687
Full Name hyaluronan and proteoglycan link protein 4
Calculated Molecular Weight 402 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC142644
Gene Symbol HAPLN4
Gene ID (NCBI) 404037
RRIDAB_10695504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HAPLN4 antibody 21228-1-APDownload protocol
IHC protocol for HAPLN4 antibody 21228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Diabetes

Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.

Authors - Yuguo Niu
View Article