HARS Polyclonal antibody

HARS Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16375-1-AP

Synonyms

HARS, HisRS, Histidine tRNA ligase, histidyl tRNA synthetase, HRS



Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, human kidney tissue, K-562 cells, mouse brain tissue, mouse heart tissue, mouse lung tissue
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16375-1-AP targets HARS in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HARS fusion protein Ag9333
Full Name histidyl-tRNA synthetase
Calculated Molecular Weight 509 aa, 57 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC011807
Gene Symbol HARS
Gene ID (NCBI) 3035
RRIDAB_2116283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Protocols

Product Specific Protocols
WB protocol for HARS antibody 16375-1-APDownload protocol
IF protocol for HARS antibody 16375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.

Authors - Maria C Passarelli