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HBG1/2-Specific Polyclonal antibody

HBG1/2-Specific Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 19993-1-AP

Synonyms

Gamma 1 globin, Hb F Agamma, HBG1, HBG1-Specific, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using HBG1/2-Specific Polyclonal antibody (19993-1-AP)

    WB analysis of human placenta using 19993-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19993-1-AP (HBG1/2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of recombinant proein using HBG1/2-Specific Polyclonal antibody (19993-1-AP)

    WB analysis of recombinant proein using 19993-1-AP

    WB results of 19993-1-AP (HBG1/2-Specific antibody) at dilution of 1:2000.

  • Flow cytometry (FC) experiment of K-562 cells using HBG1/2-Specific Polyclonal antibody (19993-1-AP)

    FC experiment of K-562 using 19993-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human HBG1/2-Specific (19993-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inrecombinant proein, human placenta tissue
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19993-1-AP targets HBG1/2-Specific in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name hemoglobin, gamma A
Calculated Molecular Weight 16 kDa
GenBank Accession NumberNM_000559
Gene Symbol HBG1
Gene ID (NCBI) 3047
RRIDAB_10949629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HBG1, also named as Hb F Agamma, HBGA, HBGR and HSGGL, belongs to the globin family. HBG1 makes up the fetal hemoglobin F, in combination with alpha chains. Some gamma variants can cause severe jaundice and cyanosis in premature and new born babies. The antibody is specific to HBG1 and HBG2. The antibody has no cross reaction to HBB and HBD. HBG1 protein migrates around 16 kDa. Heterodimer of 32 kDa may also be observed. This antibody could identify HBG1 and HBG2.

Protocols

Product Specific Protocols
WB protocol for HBG1/2-Specific antibody 19993-1-APDownload protocol
FC protocol for HBG1/2-Specific antibody 19993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols