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HBG1/2-Specific Monoclonal antibody

HBG1/2-Specific Monoclonal Antibody for FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2A4B5

Cat no : 66168-1-Ig

Synonyms

Gamma 1 globin, Hb F Agamma, HBG1, HBG1-Specific, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using HBG1/2-Specific Monoclonal antibody (66168-1-Ig)

    IHC staining of human placenta using 66168-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66168-1-Ig (HBG1/2-Specific antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HBG1-Specific Monoclonal antibody (66168-1-Ig)

    IHC staining of human placenta using 66168-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66168-1-Ig (HBG1-Specific antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HBG1/2-Specific Monoclonal antibody (66168-1-Ig)

    IHC staining of human placenta using 66168-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66168-1-Ig (HBG1/2-Specific antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of K-562 cells using HBG1/2-Specific Monoclonal antibody (66168-1-Ig)

    FC experiment of K-562 using 66168-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human HBG1/2-Specific (66168-1-Ig, Clone:2A4B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

Tested Applications

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:16000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66168-1-Ig targets HBG1/2-Specific in IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name hemoglobin, gamma A
Calculated Molecular Weight 16 kDa
GenBank Accession NumberNM_000559
Gene Symbol HBG1
Gene ID (NCBI) 3047
RRIDAB_2881564
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HBG1, also named as Hb F Agamma, HBGA, HBGR and HSGGL, belongs to the globin family. HBG1 makes up the fetal hemoglobin F, in combination with alpha chains. Some gamma variants can cause severe jaundice and cyanosis in premature and new born babies. The antibody is specific to HBG1 and HBG2. The antibody has no cross reaction to HBB and HBD. HBG1 protein migrates around 16 kDa. Heterodimer of 32 kDa may also be observed. This antibody could identify HBG1 and HBG2.

Protocols

Product Specific Protocols
IHC protocol for HBG1/2-Specific antibody 66168-1-IgDownload protocol
FC protocol for HBG1/2-Specific antibody 66168-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols