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HELT Polyclonal antibody

HELT Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 24730-1-AP

Synonyms

HCM1228, HELT, HESL, Mgn

Validation Data Gallery

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  • Western Blot (WB) analysis of rat heart tissue using HELT Polyclonal antibody (24730-1-AP)

    WB analysis of rat heart using 24730-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 24730-1-AP (HELT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using HELT Polyclonal antibody (24730-1-AP)

    WB analysis of mouse heart using 24730-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 24730-1-AP (HELT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inrat heart tissue, mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24730-1-AP is HELT Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HELT fusion protein Ag20618
Full Name HES/HEY-like transcription factor
Calculated Molecular Weight 327 aa, 36 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC140795
Gene Symbol HELT
Gene ID (NCBI) 391723
RRIDAB_2879694
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HELT is a transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'. HELT has two isoforms with MW 36 kDa and 27 kDa.

Protocols

Product Specific Protocols
WB protocol for HELT antibody 24730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols