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HEYL Polyclonal antibody

HEYL Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15679-1-AP

Synonyms

bHLHb33, HEY3, HEYL, hHeyL, hHRT3, HRT 3, HRT3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEYL Polyclonal antibody (15679-1-AP)

    WB analysis using 15679-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15679-1-AP (HEYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using HEYL Polyclonal antibody (15679-1-AP)

    WB analysis of MDA-MB-453s using 15679-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15679-1-AP (HEYL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HEYL Polyclonal antibody (15679-1-AP)

    IP experiment of HeLa using 15679-1-AP

    IP result of anti-HEYL (IP:15679-1-AP, 4ug; Detection:15679-1-AP 1:300) with HeLa cells lysate 2600ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HEYL Polyclonal antibody (15679-1-AP)

    IF Staining of HeLa using 15679-1-AP

    Immunofluorescent analysis of Hela cells, using HEYL antibody 15679-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inMDA-MB-453s cells, HeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

15679-1-AP targets HEYL in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEYL fusion protein Ag8163
Full Name hairy/enhancer-of-split related with YRPW motif-like
Calculated Molecular Weight 328 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC006087
Gene Symbol HEYL
Gene ID (NCBI) 26508
RRIDAB_2878167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEYL, also named as Hairy/enhancer-of-split related with YRPW motif-like protein, is a 328 amino acid protein, which belongs to the HEY family. HEYL as downstream effector of Notch signaling may be required for cardiovascular development. HEYL represses transcription by the cardiac transcriptional activators GATA4 and GATA6.

Protocols

Product Specific Protocols
WB protocol for HEYL antibody 15679-1-APDownload protocol
IF protocol for HEYL antibody 15679-1-APDownload protocol
IP protocol for HEYL antibody 15679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Adv Sci (Weinh)

Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner

Authors - Hao Sun
View Article