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HGD Polyclonal antibody

HGD Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16465-1-AP

Synonyms

AKU, HGD, HGO, Homogentisate 1,2 dioxygenase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HGD Polyclonal antibody (16465-1-AP)

    WB analysis using 16465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16465-1-AP (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HGD Polyclonal antibody (16465-1-AP)

    WB analysis using 16465-1-AP

    Rat liver tissues were subjected to SDS PAGE followed by western blot with 16465-1-AP (HGD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HGD Polyclonal antibody (16465-1-AP)

    WB analysis of HeLa using 16465-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16465-1-AP (HGD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using HGD Polyclonal antibody (16465-1-AP)

    IHC staining of human liver using 16465-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16465-1-AP (HGD antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using HGD Polyclonal antibody (16465-1-AP)

    IHC staining of human liver using 16465-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16465-1-AP (HGD antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16465-1-AP targets HGD in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HGD fusion protein Ag9544
Full Name homogentisate 1,2-dioxygenase (homogentisate oxidase)
Calculated Molecular Weight 37 kDa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC020792
Gene Symbol HGD
Gene ID (NCBI) 3081
RRIDAB_2248397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).

Protocols

Product Specific Protocols
WB protocol for HGD antibody 16465-1-APDownload protocol
IHC protocol for HGD antibody 16465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols