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HIBCH Polyclonal antibody

HIBCH Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14603-1-AP

Synonyms

HIB CoA hydrolase, HIBCH, HIBYL COA H

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIBCH Polyclonal antibody (14603-1-AP)

    WB analysis using 14603-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14603-1-AP (HIBCH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using HIBCH Polyclonal antibody (14603-1-AP)

    WB analysis of human brain using 14603-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14603-1-AP (HIBCH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using HIBCH Polyclonal antibody (14603-1-AP)

    WB analysis of human liver using 14603-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14603-1-AP (HIBCH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using HIBCH Polyclonal antibody (14603-1-AP)

    WB analysis of human heart using 14603-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14603-1-AP (HIBCH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HIBCH Polyclonal antibody (14603-1-AP)

    IHC staining of human liver cancer using 14603-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14603-1-AP (HIBCH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HIBCH Polyclonal antibody (14603-1-AP)

    IHC staining of human liver cancer using 14603-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14603-1-AP (HIBCH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, human brain tissue, human liver tissue, human heart tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14603-1-AP targets HIBCH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIBCH fusion protein Ag6149
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC067822
Gene Symbol HIBCH
Gene ID (NCBI) 26275
RRIDAB_2116843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

Protocols

Product Specific Protocols
WB protocol for HIBCH antibody 14603-1-APDownload protocol
IHC protocol for HIBCH antibody 14603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Hum Genet

A Common Polymorphism in HIBCH Influences Methylmalonic Acid Concentrations in Blood Independently of Cobalamin.

Authors - Anne M Molloy
View Article