HLA-DPA1 Polyclonal antibody
HLA-DPA1 Polyclonal Antibody for WB, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16109-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Raji cells, mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
16109-1-AP targets HLA-DPA1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HLA-DPA1 fusion protein Ag9031 |
| Full Name | major histocompatibility complex, class II, DP alpha 1 |
| Calculated Molecular Weight | 260 aa, 29 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC009956 |
| Gene Symbol | HLA-DPA1 |
| Gene ID (NCBI) | 3113 |
| RRID | AB_1851387 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HLA-DPA1 antibody 16109-1-AP | Download protocol |
| IHC protocol for HLA-DPA1 antibody 16109-1-AP | Download protocol |
| FC protocol for HLA-DPA1 antibody 16109-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci Activation of GABA receptor attenuates intestinal inflammation by modulating enteric glial cells function through inhibiting NF-κB pathway | ||
Aging (Albany NY) HLA-DPA1 overexpression inhibits cancer progression, reduces resistance to cisplatin, and correlates with increased immune infiltration in lung adenocarcinoma | ||
iScience Single-cell transcriptomics reveals the role of antigen presentation in liver metastatic breast cancer | ||
Front Immunol Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics |
