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HLA-DRB1 Polyclonal antibody

HLA-DRB1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 15862-1-AP

Synonyms

DR4, DRB1, DW2.2/DR2.2, HLA DR1B, HLA DRB, HLA DRB1, HLA DRB1*, HLA DRB2, HLA-DRB1, MHC class II antigen DRB1*15, SS1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA-DRB1 Polyclonal antibody (15862-1-AP)

    WB analysis using 15862-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using HLA-DRB1 Polyclonal antibody (15862-1-AP)

    WB analysis of A375 using 15862-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using HLA-DRB1 Polyclonal antibody (15862-1-AP)

    WB analysis of Raji using 15862-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using HLA-DRB1 Polyclonal antibody (15862-1-AP)

    WB analysis of human spleen using 15862-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using HLA-DRB1 Polyclonal antibody (15862-1-AP)

    IP experiment of Raji using 15862-1-AP

    IP result of anti-HLA-DRB1 (IP:15862-1-AP, 4ug; Detection:15862-1-AP 1:500) with Raji cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DRB1 Polyclonal antibody (15862-1-AP)

    IHC staining of human tonsillitis using 15862-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DRB1 Polyclonal antibody (15862-1-AP)

    IHC staining of human tonsillitis using 15862-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Raji cells using HLA-DRB1 Polyclonal antibody (15862-1-AP)

    FC experiment of Raji using 15862-1-AP

    1X10^6 Raji cells were stained with 0.20ug HLA-DRB1 antibody (15862-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

Tested Applications

Positive WB detected inRaji cells, human spleen tissue, A375 cells, Daudi cells
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

15862-1-AP targets HLA-DRB1 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DRB1 fusion protein Ag8405
Full Name major histocompatibility complex, class II, DR beta 1
Calculated Molecular Weight 266 aa, 30 kDa
Observed Molecular Weight 27-30 kDa
GenBank Accession NumberBC007920
Gene Symbol HLA-DRB1
Gene ID (NCBI) 3123
RRIDAB_1639897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
IHC protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
IP protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
FC protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation.

Authors - Semini Geo G
View Article
humanWB

Nat Commun

Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes

Authors - Rupert L Mayer
View Article
mouseWB

Heliyon

Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation

Authors - Qian Gao
View Article