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HLA-DRB3 Polyclonal antibody

HLA-DRB3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16789-1-AP

Synonyms

HLA DR3B, HLA DRB3, HLA-DRB3, MHC class II antigen DRB3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA-DRB3 Polyclonal antibody (16789-1-AP)

    WB analysis using 16789-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16789-1-AP (HLA-DRB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16789-1-AP targets HLA-DRB3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DRB3 fusion protein Ag10223
Full Name major histocompatibility complex, class II, DR beta 3
Calculated Molecular Weight 266 aa, 30 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC008987
Gene Symbol HLA-DRB3
Gene ID (NCBI) 3125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are heterodimers consisting of an alpha (DRA) and a beta (DRB) chain. HLA-DRB3 is a beta chain of MHC class II molecule expressed on the surface of antigen presenting cells (APC) like B cells, macrophages, and dendritic cells (PMID: 30192820). It is involved in adaptive immune responses by presenting peptide antigens to CD4+ T cells (PMID: 19830726).

Protocols

Product Specific Protocols
WB protocol for HLA-DRB3 antibody 16789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols