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HLA-DRB5 Polyclonal antibody

HLA-DRB5 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 21702-1-AP

Synonyms

DR beta 2, DR beta 5, Dw2, HLA DRB, HLA DRB5, HLA-DRB5, MHC class II antigen DRB5

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using HLA-DRB5 Polyclonal antibody (21702-1-AP)

    WB analysis of HepG2 using 21702-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using HLA-DRB5 Polyclonal antibody (21702-1-AP)

    WB analysis of Raji using 21702-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HLA-DRB5 Polyclonal antibody (21702-1-AP)

    WB analysis of HeLa using 21702-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using HLA-DRB5 Polyclonal antibody (21702-1-AP)

    WB analysis of HL-60 using 21702-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DRB5 Polyclonal antibody (21702-1-AP)

    IHC staining of human tonsillitis using 21702-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DRB5 Polyclonal antibody (21702-1-AP)

    IHC staining of human tonsillitis using 21702-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DRB5 Polyclonal antibody (21702-1-AP)

    IHC staining of human tonsillitis using 21702-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DRB5 Polyclonal antibody (21702-1-AP)

    IHC staining of human tonsillitis using 21702-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Raji cells using HLA-DRB5 Polyclonal antibody (21702-1-AP)

    FC experiment of Raji using 21702-1-AP

    1X10^6 Raji cells were stained with 0.20ug HLA-DRB5 antibody (21702-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HL-60 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21702-1-AP is HLA-DRB5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DRB5 fusion protein Ag16307
Full Name major histocompatibility complex, class II, DR beta 5
Calculated Molecular Weight 266 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC009234
Gene Symbol HLA-DRB5
Gene ID (NCBI) 3127
RRIDAB_2878905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HLA-DRB5 antibody 21702-1-APDownload protocol
IHC protocol for HLA-DRB5 antibody 21702-1-APDownload protocol
FC protocol for HLA-DRB5 antibody 21702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols