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  • Featured Product
  • KD/KO Validated

HLX Polyclonal antibody

HLX Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14336-1-AP

Synonyms

H2.0 like homeobox, H2.0 like homeobox protein, HB24, HLX, HLX1, Homeobox protein HB24, Homeobox protein HLX1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using HLX Polyclonal antibody (14336-1-AP)

    WB analysis of human placenta using 14336-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14336-1-AP (HLX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using HLX Polyclonal antibody (14336-1-AP)

    IHC staining of human endometrial cancer using 14336-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14336-1-AP (HLX antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using HLX Polyclonal antibody (14336-1-AP)

    IHC staining of human endometrial cancer using 14336-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14336-1-AP (HLX antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HLX Polyclonal antibody (14336-1-AP)

    IF Staining of A549 using 14336-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 14336-1-AP (HLX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14336-1-AP targets HLX in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLX fusion protein Ag5627
Full Name H2.0-like homeobox
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC033808
Gene Symbol HLX
Gene ID (NCBI) 3142
RRIDAB_2232971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In immune system, Hlx is a Th1-special transcription factor that interacts specifically with T-bet, and synergistically they promote IFN-γ expression when co-expressed. It can down-regulate the IL-4 receptor expression in naive CD4+ T cells [PMID:14688316]. Over expression of Hlx contributes ot the aberrant expression of IFN-γ in normal CD4+ T cells during the differentiation under Th2-polarizing conditions. In addition, Hlx is a negative regulator of IFN-γ production in NK cells and its inhibitory function is achieved at least in part through the proteasomal degradation of STAT4 [PMID:16424184].

Protocols

Product Specific Protocols
WB protocol for HLX antibody 14336-1-APDownload protocol
IHC protocol for HLX antibody 14336-1-APDownload protocol
IF protocol for HLX antibody 14336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Tumour Biol

H2.0-like homeobox 1 acts as a tumor suppressor in hepatocellular carcinoma.

Authors - Ting Liu
  • KD Validated
View Article