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HMGA1 Polyclonal antibody

HMGA1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15398-1-AP

Synonyms

high mobility group AT hook 1, High mobility group protein A1, High mobility group protein R, HMG I(Y), HMG R, HMGA1, HMGA1A, HMGIY

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using HMGA1 Polyclonal antibody (15398-1-AP)

    WB analysis of COLO 320 using 15398-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15398-1-AP (HMGA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HMGA1 Polyclonal antibody (15398-1-AP)

    IHC staining of human prostate cancer using 15398-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15398-1-AP (HMGA1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15398-1-AP targets HMGA1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited ReactivityHamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGA1 fusion protein Ag7613
Full Name high mobility group AT-hook 1
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC004924
Gene Symbol HMGA1
Gene ID (NCBI) 3159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Publications

SpeciesApplicationTitle
HamsterWB

Lipids

The Safety and Anti-Hypercholesterolemic Effect of Coptisine in Syrian Golden Hamsters.

Authors - Kai He
View Article